Flow Cytometry from Fundamentals to Advanced Applications
Whether you're working in clinical diagnostics, therapeutic development, or research applications, this training provides the technical depth and FDA regulatory awareness essential for success in today's competitive biotech environment.
Transform Your Career
Transform Your Career
- From immunophenotyping to multi-parameter assays
- Master assay validation, documentation, and audit readiness
- Prepare for IND/BLA submissions and lead high-impact flow cytometry programs
Build Expertise from the Ground Up
Core concepts:
Real-World Applications
Specialized content for cell and gene therapy:
Core concepts:
- Principles of Flow Cytometry: Hydrodynamic focusing, optics, fluidics, electronics, and fluorochrome behavior
- Fluorophore Spectral Properties: Understanding excitation/emission profiles, Stokes shift, and spillover
- Panel Design Fundamentals: Antigen density, co-expression patterns, fluorochrome brightness, and spectral separation
- Compensation & Spectral Unmixing: Theory and best practices across conventional and spectral cytometers
- Gating Strategies: Boolean logic, hierarchical gating, exclusion/inclusion gating, and population identification
- Quality Control & Assurance: Daily instrument QC, performance metrics (e.g., CVs, rCVs, stain index), and troubleshooting
- Clinical & Regulatory Requirements: CLIA, CAP, and FDA expectations for method validation, documentation, and data integrity
- FlowJo for gating, panel design, and data analysis
- Instrument Setup & Calibration: PMT voltage optimization, laser alignment, and baseline setup using beads or reference controls
- Multicolor Panel Design: Designing 6–20+ color panels for immunophenotyping, cytokine profiling, MRD, and CAR expression
- Sample Preparation & Staining: Surface, intracellular, viability, barcoding, and fixation/permeabilization protocols
- Data Acquisition: Best practices for event collection, thresholding, doublet discrimination, and acquisition templates
- Data Analysis: Manual gating, t-SNE/UMAP, FlowSOM, and high-dimensional analysis Assay Validation: For various regulatory environments like RUO, GMP, GLP, CLIA, IVD: Accuracy, precision, specificity, LOD/LOQ, linearity, and inter/intra-assay reproducibility
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Documentation & Compliance: Creating SOPs, validation reports, assay protocols, and audit- ready records for FDA and CAP compliance
Real-World Applications
Specialized content for cell and gene therapy:
- CAR-T monitoring
- Minimal residual disease (MRD) analysis FDA & ICH Q2-aligned validation strategies
- Build and validate GxP-compliant flow cytometry assays for identity, purity, viability, and potency testing with suggested pre-made panels
- Design scalable, phase-appropriate assays to support IND, BLA, and commercial release of CGT products
- Train staff on assay transfer, qualification, and lifecycle management for client- sponsored programs
- Detect and quantify CAR expression, vector transduction efficiency, and residual impurities
- Optimize high-parameter panel design (18–30+ colors) for in-process monitoring and QC lot release
- FACS sorting for single-cell workflows Companion diagnostics development
- ML tools (tSNE, UMAP) for automated classification
Format
Live Virtual
Starting date
October 14
Duration
6 Weeks:
Different Lectures every Tuesday and Thursday
Early Bird Price
$2040
$2400 Regular
Accreditation
CMLE Credit Granted by ASCP
Accreditation
12 Credits of CMLE Hours
Write your awesome label here.
Live Sessions
Engage with our SME instructor and other professionals in real-time.
Assessments
Your knowledge will be activated and added to your toolkit through assessments and discussions.
PowerPoints Versions Included
Access the learning material in traditional PPT versions for self-paced reviews.
Professional Networking
Meet other like-minded professionals during courses and our communities.
Course Components
